FastQCFastQC Report
Thu 16 Oct 2025
HPAP-075_beta_5000126_NSS0011_s_1_GCCTTAAC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-075_beta_5000126_NSS0011_s_1_GCCTTAAC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20287215
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG364720.17977824950344343No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT350430.1727344044019842No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT319010.15724681776182683No Hit
AGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGT312230.15390481147855928No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT263880.13007206755584738No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTC248450.1224662921943697No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG239870.11823702760580986No Hit
GGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGG226680.11173539591314036No Hit
AGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAG206560.10181781974509561No Hit
TGGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTT206130.10160586359438689No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG205310.1012016681441982No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA203160.10014188739065466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)