FastQCFastQC Report
Thu 16 Oct 2025
HPAP-075_beta_5000126_NSS0011_s_2_GCCTTAAC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-075_beta_5000126_NSS0011_s_2_GCCTTAAC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19721424
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG356990.18101634040219408No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT337050.17090550864886836No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT309830.15710325988630436No Hit
AGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGT303600.15394425879186005No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT262220.13296200112121723No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTC237550.12045276243743859No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG228370.11579792615381121No Hit
GGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGG218300.11069180399954892No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG200600.10171679286445036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)