FastQCFastQC Report
Thu 16 Oct 2025
HPAP-111_beta_5000006_NSS0004_s_2_CAGGTTCA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-111_beta_5000006_NSS0004_s_2_CAGGTTCA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19582788
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG654550.33424760560140876No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT601630.3072238743533352No Hit
GGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGG364260.18601028617579887No Hit
AAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGA362390.1850553659672974No Hit
AGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGT341180.17422442606231553No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT300640.15352257298603242No Hit
AGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAG263720.1346692820246024No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTC259030.13227432171558No Hit
AGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAG255030.13023171164391914No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC248560.12692778985300765No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA246980.12612095887470162No Hit
GTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGT223610.11418700953102286No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT222660.11370188963900339No Hit
TGGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTT217430.11103117697030679No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG201160.1027228605038261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)