FastQCFastQC Report
Thu 16 Oct 2025
HPAP-116_alpha_5000008_NSS0004_s_1_TACTCCAG_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-116_alpha_5000008_NSS0004_s_1_TACTCCAG_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17311427
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT631210.3646204325039178No Hit
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC355090.20511885010981476No Hit
GGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGT249910.144361293843656No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT238550.13779915428115777No Hit
CTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTTGCCAG221250.12780575512348No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC202400.11691699361352476No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA185820.10733950470980815No Hit
CTCAGAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCA177120.1023139224744442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)