FastQCFastQC Report
Thu 16 Oct 2025
HPAP-118_alpha_5000013_NSS0004_s_2_TTGCGAGA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-118_alpha_5000013_NSS0004_s_2_TTGCGAGA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19051660
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC576220.3024513349492905No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT439070.23046285730482277No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA391930.2057196065854629No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC387230.20325262995455512No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC298300.15657428276591123No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT266460.13986182831312335No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG249440.13092822357736808No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG247010.12965274417032427No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT243560.127841878345509No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC237920.12488150638841969No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG209950.1102003709912942No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG201450.10573881750986529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)