FastQCFastQC Report
Thu 16 Oct 2025
HPAP-120_beta_5000017_NSS0004_s_2_TCGGATTC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-120_beta_5000017_NSS0004_s_2_TCGGATTC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18820803
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG381220.20255246282531092No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT333770.1773409986810871No Hit
AAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGA216920.1152554436704959No Hit
AGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGT210340.11175931228864147No Hit
GGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGG198570.10550559399617541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)