FastQCFastQC Report
Thu 16 Oct 2025
HPAP-120_exocrine_5000018_NSS0004_s_2_CGAATTGC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-120_exocrine_5000018_NSS0004_s_2_CGAATTGC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28675128
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC869400.3031895794850506No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG802240.27976858551424777No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA647960.2259658614252742No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT644790.22486037377060708No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC616000.21482031396686357No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG604510.21081335713654006No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT564160.19674192910315866No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG544810.18999392086410216No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA518030.18065481695495833No Hit
GGGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAG495460.17278388434743866No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC485510.16931397830203235No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC480620.1676086676927824No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG436210.15212137849916485No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT418290.14587206027467425No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT410080.14300895186936916No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC407080.14196274904160847No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT400160.13954950785224046No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC353840.12339613619161527No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG353790.12337869947781925No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT350680.12229413587970732No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG348160.12141532550438833No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG339560.11841621073147433No Hit
GGCGGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACT336600.11738395727475044No Hit
CAGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCT319490.11141711381375526No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG315960.11018608181975684No Hit
GGAGGCTTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCA314250.10958974620793323No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG311430.10860631554983817No Hit
TGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGG305270.10645811241016953No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC296720.10347643435105154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)