FastQCFastQC Report
Thu 16 Oct 2025
HPAP-121_beta_5000020_NSS0004_s_1_GTCCTTGA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-121_beta_5000020_NSS0004_s_1_GTCCTTGA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22500182
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC1108270.492560460177611No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC886600.39404125708849824No Hit
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG760120.3378283784548943No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG595730.26476674722008914No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT512690.22786037908493362No Hit
AAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGA411810.18302518619627167No Hit
AGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGT359450.15975426332107No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT359010.15955870934732885No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG351000.15599873814354034No Hit
GGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGG319280.1419010744001982No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA282730.12565676135419704No Hit
AGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAG270220.12009680632805549No Hit
GTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGT264850.11771015896671413No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC264740.11766127047327885No Hit
GCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGAT264420.11751904940146705No Hit
GGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGCC246990.10977244539621947No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTC238420.10596358731676037No Hit
AGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAG233890.10395027026892494No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG226810.10080362905508942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)