FastQCFastQC Report
Thu 16 Oct 2025
HPAP-121_exocrine_5000021_NSS0004_s_1_GGAGGAAT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-121_exocrine_5000021_NSS0004_s_1_GGAGGAAT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33487872
Sequences flagged as poor quality0
Sequence length20-100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC6086821.8176192264471152No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC4925471.4708220337201479No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG2008780.5998529855823624No Hit
GGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGCC1391530.41553252473014707No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG1175310.350965866090267No Hit
GGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGCCGA1105120.3300060392012965No Hit
CGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCG997630.29790785153502736No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA946210.28255303890315875No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT903300.26973944477570866No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC889750.2656932037962878No Hit
GGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGCCG822620.24564714055285447No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC733610.21906736862826037No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT710380.21213052892700973No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC662800.19792240008561904No Hit
GCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCG603710.18027720602849892No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG592530.17693868395101367No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT570590.1703870583356267No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG554200.16549274913616488No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC550880.16450134544231415No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG549250.1640146020625019No Hit
CCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCC529090.15799451216249272No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT503480.15034696740360212No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA495460.1479520705286977No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG483780.14446424066599395No Hit
CGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCG451670.13487569469926305No Hit
GGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGCCGAG450950.13466069148854845No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG436990.13049201812524847No Hit
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG417160.12457047136348347No Hit
TTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTC386430.11539401488395559No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC369320.11028470247377915No Hit
CCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCG367120.10962774821881784No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG354620.10589505358835581No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA348180.10397196931474176No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT338570.10110227368284257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)