FastQCFastQC Report
Thu 16 Oct 2025
HPAP-122_alpha_5000022_NSS0004_s_1_AGGAACAC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-122_alpha_5000022_NSS0004_s_1_AGGAACAC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15618470
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG655740.4198490633205429No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT450090.28817803536453956No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC428280.2742137994310582No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG391110.2504150534591416No Hit
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC335240.21464330372949464No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC318430.20388040569914978No Hit
GGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGT221990.14213300022345338No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC217080.13898928640257335No Hit
CTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAATG210560.13481474177688338No Hit
CTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTTGCCAG196720.12595343846100163No Hit
CTCAGAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCA191190.12241275874013267No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT168300.10775703381957387No Hit
CTGCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGG163450.1046517360535315No Hit
GGGAACGTTGCCAGCTGCCTTGTACCAGCATTACAAATAATCCAGCCACA157880.10108544562943746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)