FastQCFastQC Report
Thu 16 Oct 2025
HPAP-122_alpha_5000022_NSS0004_s_2_AGGAACAC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-122_alpha_5000022_NSS0004_s_2_AGGAACAC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16002176
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG682320.42639201068654664No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT464500.29027302286888984No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC440960.27556252349680443No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG402730.25167202260492577No Hit
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC342240.21387091355575644No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC327990.20496587464104882No Hit
GGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGT232340.14519275378548518No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC222020.13874363086620217No Hit
CTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAATG218330.13643769447355159No Hit
CTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTTGCCAG199100.12442057880128303No Hit
CTCAGAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCA196640.1228832878728493No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT177270.11077868409896253No Hit
CTGCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGG167830.10487948638985098No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG164140.10257354999720038No Hit
GGGAACGTTGCCAGCTGCCTTGTACCAGCATTACAAATAATCCAGCCACA161540.10094877096714847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)