FastQCFastQC Report
Thu 16 Oct 2025
HPAP-124_alpha_5000028_NSS0004_s_1_CTTCGGTT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-124_alpha_5000028_NSS0004_s_1_CTTCGGTT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10634068
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC641790.6035225654001836No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG494430.46494906746881814No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC477030.4485865616055869No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG284640.2676680269488591No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT283440.2665395782686362No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG177340.1667659074589329No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC158910.1494348164785104No Hit
GGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGCC152720.14361390203636087No Hit
GGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGCCGA129410.1216937864230321No Hit
CGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCG106930.10055418114685744No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG106670.10030968393280916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)