FastQCFastQC Report
Thu 16 Oct 2025
HPAP-124_exocrine_5000030_NSS0004_s_1_CTCGAACA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-124_exocrine_5000030_NSS0004_s_1_CTCGAACA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15986838
Sequences flagged as poor quality0
Sequence length20-100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC780550.4882453928663067No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG754700.4720758413890226No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG737320.4612043982681253No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG523710.3275882322695708No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC384010.24020384769020617No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA382770.23942820963094766No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG364850.22821898864553453No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC360230.2253291113602327No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG342960.2145264748413664No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT319150.1996329730744754No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT297090.18583412179444114No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA280370.17537551828572978No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC279930.1751002918776058No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT277590.17363658779803737No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC269590.1686324712866922No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC265570.16611790273974128No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG238680.14929781611598242No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT238260.14903509999913678No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG236630.14801551125995022No Hit
GGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTAC234010.14637666310248468No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC228830.1431364976613887No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG227910.14256102426258402No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT213310.1334285116293791No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG205520.1285557531764568No Hit
CGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGCG202980.1269669461841047No Hit
GGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGCC201510.12604743977514501No Hit
GCGGACACTGTCTGGGAGAAGCGGGCAGGTGTGGCCAGCTTCAGCAGGGT200860.12564085530859823No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG199540.12481517608422629No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC193500.12103706811816071No Hit
GGGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTA193500.12103706811816071No Hit
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG192330.12030521607837648No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC189770.11870389879474602No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA189160.11832233491075597No Hit
GTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTACCCTC183890.11502587315890736No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG181990.11383739548746288No Hit
CCATAGTTGAACTTGGTCCCGTAGAGAGAGGCCAGGGCTGTCACAGCAGC181560.11356842422497807No Hit
GGGAAGGGCACTCGGACGTCGATGGGGGAGCCAGGGTGGGCAGGCCCCCG181520.11354340364242135No Hit
TTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTC180550.11293665451542075No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG180140.1126801935442143No Hit
GGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACCTGGTAGGGG172770.1080701512081376No Hit
TGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGG169990.10633122072044515No Hit
GGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGCCCG168040.10511146732080479No Hit
CTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCGGAGACTCGAGTGAA166370.10406685799906149No Hit
GTGATTTCCAAAAATCAAGCTGGAGATGTTCTTGAGCCTCCAATTGTAGC164250.10274076712355501No Hit
GGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGCCGA160320.10028249488735672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)