FastQCFastQC Report
Thu 16 Oct 2025
HPAP-124_exocrine_5000030_NSS0004_s_2_CTCGAACA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-124_exocrine_5000030_NSS0004_s_2_CTCGAACA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16371550
Sequences flagged as poor quality0
Sequence length20-100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC799250.4881944592906597No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG780230.4765767444133268No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG758740.4634503147227966No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG534750.32663370297864286No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC399540.24404531030965304No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA390560.23856018519932443No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC377610.230650121705031No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG373030.22785258573562064No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG352120.21508042915912057No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT327310.19992609129862474No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT307330.18772199333600056No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA288780.1763913618441748No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT287300.17548735458768414No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC284300.17365490744614898No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC278090.16986174186317116No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC276920.16914708747797247No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG244290.14921617073520832No Hit
GGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTAC243730.1488741139354551No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG243490.1487275181641323No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT242860.1483427042644099No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC236280.14432353686730945No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG235050.14357223353928003No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT216840.1324492793901616No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG211230.12902260323549083No Hit
CGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGCG208570.127397833436663No Hit
GGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCCGCCGCCGCGCGCC206290.12600517360909627No Hit
GCGGACACTGTCTGGGAGAAGCGGGCAGGTGTGGCCAGCTTCAGCAGGGT205900.1257669554806967No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG205520.12553484550943558No Hit
GGGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTA200350.12237692826885666No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC198430.12120416209827414No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC196990.12032458747033725No Hit
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG196850.12023907327039896No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA193850.1184066261288638No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG189590.11580455118788387No Hit
TTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTC187560.1145645952887784No Hit
CCATAGTTGAACTTGGTCCCGTAGAGAGAGGCCAGGGCTGTCACAGCAGC186800.11410037534625617No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG186770.1140820508748408No Hit
GGGAAGGGCACTCGGACGTCGATGGGGGAGCCAGGGTGGGCAGGCCCCCG186660.11401486114631786No Hit
GTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTACCCTC185110.113068096789858No Hit
GGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACCTGGTAGGGG182350.11138224541964567No Hit
TGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGG173270.10583603873793258No Hit
GGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGCCCG172250.10521300670981062No Hit
CTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCGGAGACTCGAGTGAA171190.10456554205313486No Hit
GTGATTTCCAAAAATCAAGCTGGAGATGTTCTTGAGCCTCCAATTGTAGC166950.10197568342643183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)