FastQCFastQC Report
Thu 16 Oct 2025
HPAP-125_exocrine_5000033_NSS0004_s_1_AGCTACCA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-125_exocrine_5000033_NSS0004_s_1_AGCTACCA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18328371
Sequences flagged as poor quality0
Sequence length20-100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG1267270.6914253317984451No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG979980.5346792685503802No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC459780.25085699105501524No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG393830.21487452430988002No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG379780.2072088130472697No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC373150.20359147029487779No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT370220.20199285577534415No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG370060.20190555941932867No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA345080.1882764158364101No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC296840.1619565644977396No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA289240.1578099875870038No Hit
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG280850.1532323849184415No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC279970.15275225496035627No Hit
CGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAG268600.14654875766100545No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC264290.14419721207083816No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC244180.13322515132414114No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT231390.12624689886515283No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG230380.12569584061780506No Hit
CTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCGG227330.12403175383125975No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC227240.12398264963100103No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG222770.12154380768481825No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT221920.12108004579348594No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG221160.12066538810241237No Hit
GGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCAGTGAG212660.11602776918908941No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT212340.11585317647705842No Hit
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTC205730.11224674576916847No Hit
GCGATAGGCATTGGTGCCTTCTGGGCAGCTGATCCGGGCCTGGGGCAACT204440.11154291889879359No Hit
GCGGACACTGTCTGGGAGAAGCGGGCAGGTGTGGCCAGCTTCAGCAGGGT203670.11112280518546902No Hit
GTCGATTTGGCGAGGGCGCTCCCGACGACGCACCGGGAGGAGGCCCTTCC195550.10669251511768285No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG194800.10628331344886023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)