FastQCFastQC Report
Thu 16 Oct 2025
HPAP-126_beta_5000035_NSS0004_s_2_CATGAGCA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-126_beta_5000035_NSS0004_s_2_CATGAGCA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30863388
Sequences flagged as poor quality0
Sequence length20-100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG2095630.679001929405806No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC1235840.400422662605933No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG1231970.39916874971730254No Hit
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG1203690.3900057893838486No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGC952240.3085338524727097No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT867240.28099313011261107No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC695410.22531874984042582No Hit
AAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGA659190.21358316203004024No Hit
AGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGT612470.19844548498693665No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG608900.1972887746478125No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT560540.1816197236674081No Hit
GGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGG547820.17749833556834393No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA484100.1568525140532206No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC477850.15482746093850747No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG472170.15298709266785615No Hit
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG447740.14507156505306548No Hit
AGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAG447540.14500676335339463No Hit
CGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAG422300.13682878885493713No Hit
GTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGT421560.1365890225661551No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTC399430.1294187144975788No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC397460.12878041775582122No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT387930.12569261676650664No Hit
AGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAG370650.12009374991494776No Hit
TGGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTT367620.11911200416493484No Hit
GCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGAT342700.11103771238595063No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG337410.10932370742965743No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT331910.10754166068870988No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT320180.10374104100301625No Hit
CGGGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAG311690.10099020885198993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)