FastQCFastQC Report
Thu 16 Oct 2025
HPAP-126_exocrine_5000036_NSS0004_s_2_CGTATCTC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-126_exocrine_5000036_NSS0004_s_2_CGTATCTC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15413741
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG820020.5320058251919505No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG693070.4496442492448783No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC397410.25782838831922766No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC354390.2299182268600465No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG307940.19978277823663962No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG305960.19849821013600785No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG286860.1861066693672873No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA249650.16196587188016198No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC195190.12663376139510843No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT194790.12637425268791008No Hit
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG191210.12405164975848497No Hit
GTCGATTTGGCGAGGGCGCTCCCGACGACGCACCGGGAGGAGGCCCTTCC186600.12106081190802415No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG180780.11728496021828835No Hit
CTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCGG154460.10020928728463778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)