FastQCFastQC Report
Thu 16 Oct 2025
HPAP-130_alpha_5000043_NSS0004_s_2_GTACACCT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-130_alpha_5000043_NSS0004_s_2_GTACACCT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17119741
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT737000.43049716698400986No Hit
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC299610.175008488738235No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT280350.1637583185399826No Hit
GGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGT267720.15638087048162702No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA217020.12676593647065104No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG194670.1137108324243924No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG187060.10926567171781396No Hit
CTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTTGCCAG178870.10448172083911784No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG177210.1035120800016776No Hit
GGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTA175960.10278192876866538No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC171200.1000015128733548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)