FastQCFastQC Report
Thu 16 Oct 2025
HPAP-130_exocrine_5000045_NSS0004_s_2_CTCGACTT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-130_exocrine_5000045_NSS0004_s_2_CTCGACTT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17515893
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT683480.39020562639883677No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG584070.3334514546303748No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG549840.31390920234554986No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC478740.27331749514569426No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA473670.27042298100359485No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC368070.21013487579537052No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT339660.19391532021804425No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT310170.177079181746543No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT271610.15506488878414593No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC270400.15437408757863502No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG262320.14976113407406633No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC260410.14867069580751607No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG260090.14848800457961234No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC246930.14097482783207227No Hit
TTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTC238510.1361677648978559No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG230410.13154339319154323No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA211220.12058762861819262No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA204090.1165170396964631No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG203600.11623729375373554No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT199450.11386801689185928No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG194430.11100204825411984No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA186890.10669738619663868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)