FastQCFastQC Report
Thu 16 Oct 2025
HPAP-131_exocrine_5000048_NSS0004_s_1_GAACGGTT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-131_exocrine_5000048_NSS0004_s_1_GAACGGTT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16465413
Sequences flagged as poor quality0
Sequence length20-100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG588210.3572397485565652No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG538850.3272617577220808No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA383750.2330643027296066No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC337520.20498726633823278No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG327140.19868314265788534No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG301530.18312932691090106No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC277450.16850473170639568No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC276110.16769090456461674No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT271180.16469674948329568No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC266670.1619576745508904No Hit
GGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTAC255430.15513124389895352No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA252830.1535521763104272No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC242440.14724197929320085No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG232170.14100466231852185No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT226080.13730600015924288No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC221220.13435435843607443No Hit
GGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCAGTGAG217570.13213759047525864No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG216980.13177926359940076No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC214300.13015160931584285No Hit
GGGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTA211620.12852395503228495No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG206810.12560267999351124No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG205600.1248678062311586No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC203180.12339805870645335No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT202180.12279072501855862No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG195260.11858797589832701No Hit
GTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTACCCTC194520.11813854896928488No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT194370.11804744891610068No Hit
GCGATAGGCATTGGTGCCTTCTGGGCAGCTGATCCGGGCCTGGGGCAACT192210.11673560815024804No Hit
GCGGACACTGTCTGGGAGAAGCGGGCAGGTGTGGCCAGCTTCAGCAGGGT186960.11354710628880066No Hit
GGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGGA185740.11280615918956907No Hit
TGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGG184060.11178583859390591No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT178230.10824508319347959No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT173160.10516590139585325No Hit
GGGAGATCTGCCAGGGCCAGCTGTGGGGCCGGGCATCCTCTCCTCCCACC173110.1051355347114585No Hit
GCAGGGTGTCTTGTTGGGAAGGATGTCACCAGCGGGAGGGAGTGAGGCGA170830.10375081390305849No Hit
CGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGCG169530.10296128010879532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)