FastQCFastQC Report
Thu 16 Oct 2025
HPAP-133_beta_5000053_NSS0004_s_1_GAATCCGT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-133_beta_5000053_NSS0004_s_1_GAATCCGT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14217951
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG207070.1456398323499638No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT189510.13328924821867794No Hit
GGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGG155040.10904524850310708No Hit
AAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGA144280.10147735070967681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)