FastQCFastQC Report
Thu 16 Oct 2025
HPAP-133_beta_5000053_NSS0004_s_2_GAATCCGT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-133_beta_5000053_NSS0004_s_2_GAATCCGT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14572285
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG212860.1460718068580185No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT196420.1347901169926336No Hit
GGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGG161120.11056605055418556No Hit
AAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGA147910.10150089707962752No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA146030.10021077682738155No Hit
AGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGAC145860.10009411701733806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)