FastQCFastQC Report
Thu 16 Oct 2025
HPAP-133_exocrine_5000146_NSS0011_s_2_CGGCATTA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-133_exocrine_5000146_NSS0011_s_2_CGGCATTA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23784434
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG1455450.6119338387451222No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT611380.25705047259060276No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA383110.1610759373126138No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG380570.16000801196278205No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC362590.15244844590373688No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT349500.14694484636464336No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA345740.1453639804924515No Hit
CCAGGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGC324680.13650944983597255No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC323940.13619832197814755No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT297610.1251280564422933No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG294260.12371957222105853No Hit
CTGGCTGTAGGTCCAGTCAATAGTGCTTCCACTGGCTTGATAAATTGCCT281460.11833790116678833No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG274170.115272871324161No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG244250.1026932152348044No Hit
CCATAGTTGAACTTGGTCCCGTAGAGAGAGGCCAGGGCTGTCACAGCAGC242840.10210039053273245No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG240430.10108712277954565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)