FastQCFastQC Report
Thu 16 Oct 2025
HPAP-135_exocrine_5000059_NSS0004_s_2_TCCTCATG_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-135_exocrine_5000059_NSS0004_s_2_TCCTCATG_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14675210
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG513850.35014831133591956No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT479060.32644166591142476No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA388790.2649297693184629No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG361550.24636785436119824No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT298020.20307716209853216No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG231150.15751052284771394No Hit
GGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTAC223820.15251570505635015No Hit
CGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCA223150.15205915281621185No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA221030.15061453975786376No Hit
GCAGGGTGTCTTGTTGGGAAGGATGTCACCAGCGGGAGGGAGTGAGGCGA218460.14886328713524372No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC217580.1482636364317785No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC197510.1345875118652476No Hit
GTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTACCCTC179140.12206980343041086No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC176410.12020952340716079No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG170500.1161823237963886No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC169950.11580754210672284No Hit
GGCGGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACT169230.11531691880388764No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT165660.11288424492732983No Hit
GAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGAT163870.11166450088278122No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT160220.10917731330590841No Hit
GGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCAGTGAG158600.10807341087452921No Hit
CAGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCT156160.10641074301492108No Hit
GTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTC155770.10614498872588535No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC153860.10484347413086423No Hit
TGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGC153370.10450957771643472No Hit
GCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAG153080.10431196555279276No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT146940.1001280390536149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)