FastQCFastQC Report
Thu 16 Oct 2025
HPAP-136_beta_5000061_NSS0004_s_1_GACGAACT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-136_beta_5000061_NSS0004_s_1_GACGAACT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13949612
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT780830.5597503357082619No Hit
GGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGG585340.4196102371879591No Hit
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG559760.4012728095949909No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT551850.3956024009843428No Hit
AGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGAC498880.35763001867005334No Hit
AAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGA406440.29136294256786494No Hit
GGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGG381900.2737710554243373No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTC356290.255412121856866No Hit
AGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGC324770.23281651131228598No Hit
CGGGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAG304190.21806341280316616No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT247740.17759633744651823No Hit
GTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGT247520.1774386269668289No Hit
AGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGT241070.17281484244866452No Hit
CTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG235410.16875738192574818No Hit
GTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCT219070.15704379447973177No Hit
AGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAG214800.15398277744212527No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA208640.1495668840108241No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC191540.13730847854406272No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC191220.13707908148269643No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC187400.1343406540626363No Hit
TTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTC185590.1330431269342832No Hit
GAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCA179860.12893548580419298No Hit
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGA177860.12750175417065365No Hit
TGGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTT169500.12150875594245919No Hit
GAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGC162680.11661973107209003No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA150160.10764457104613376No Hit
GCGGGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGA147450.10570186468268794No Hit
GCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTG143750.10304946116064016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)