FastQCFastQC Report
Thu 16 Oct 2025
HPAP-137_exocrine_5000065_NSS0004_s_1_TCCACGTT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-137_exocrine_5000065_NSS0004_s_1_TCCACGTT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17996868
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG933810.518873617342751No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT439460.24418693297078137No Hit
CGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCATT366190.20347429341594325No Hit
GGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTAC365470.20307422380383078No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC356560.1981233623539385No Hit
GCAGGGTGTCTTGTTGGGAAGGATGTCACCAGCGGGAGGGAGTGAGGCGA349800.19436715321799325No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG340250.1890606743351121No Hit
GAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGAT275090.15285437443893016No Hit
GGGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTA270790.150465069811036No Hit
CGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCA269300.14963714797485875No Hit
GGGGAATCCAGCAAAGCCATCAGGGTTGACGATGCTATCAGTGTAATATT262400.1458031475254472No Hit
GTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTACCCTC262170.14567534751046682No Hit
GTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCATTGTTCA257600.14313601677803048No Hit
CCAGGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGC253360.14078005128447907No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG251470.13972986855268374No Hit
GTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACAT249960.13889083367172556No Hit
GTGATTTCCAAAAATCAAGCTGGAGATGTTCTTGAGCCTCCAATTGTAGC240670.13372882437099612No Hit
GTTTGCAGGAGGCCTTTAATTGTGAAGAGCGCAATCAGAAAACAGATATG237410.13191739807170894No Hit
CTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCGGAGACTCGAGTGAA236730.13153955454915822No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG234700.13041158050389656No Hit
CCGGGAGATGCGCTGGAGCAGGGGAGAGATGCCCCTGCGCCTAGTGGCCT232990.12946141517512935No Hit
TGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGG232250.12905023251823597No Hit
GAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTG218850.12160449251503096No Hit
TTAAGGTTTGCAGGAGGCCTTTAATTGTGAAGAGCGCAATCAGAAAACAG218670.12150447511200281No Hit
GGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACA215930.11998198797701912No Hit
CTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCGATGTTGT214670.11928186615582223No Hit
CTTTATTCTTTATTCAGGATGTGGGATCGATCAGCACTGCCAGCTGGGCC211770.1176704746625913No Hit
GGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGGA207640.11537563091533483No Hit
GGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGCCCG194350.1079910126584248No Hit
CTTTAATTGTGAAGAGCGCAATCAGAAAACAGATATGCTGGTCAAGGAGG193170.1073353430163515No Hit
CCATAGTTGAACTTGGTCCCGTAGAGAGAGGCCAGGGCTGTCACAGCAGC187260.10405143828359467No Hit
GCGAGTTTTTCTATTTGTTTTGAAATTGGAGCCACTGATGCTTGATGAAT181600.10090644661059914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)