FastQCFastQC Report
Thu 16 Oct 2025
HPAP-137_exocrine_5000065_NSS0004_s_2_TCCACGTT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-137_exocrine_5000065_NSS0004_s_2_TCCACGTT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18441561
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG962980.5221792233314739No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT448280.24308137472744307No Hit
CGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCATT383300.20784574581294937No Hit
GGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTAC375630.2036866618829068No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC362580.19661025441392949No Hit
GCAGGGTGTCTTGTTGGGAAGGATGTCACCAGCGGGAGGGAGTGAGGCGA359360.19486419831813587No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG349230.18937117091118263No Hit
GAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGAT283790.15388610541157552No Hit
CGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCA279050.15131582407801597No Hit
GGGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTA278970.15127244380234406No Hit
GGGGAATCCAGCAAAGCCATCAGGGTTGACGATGCTATCAGTGTAATATT271910.14744413447429966No Hit
GTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTACCCTC271160.14703744438987568No Hit
GTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCATTGTTCA264660.14351279699153452No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG258690.14027554391951963No Hit
GTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACAT257490.13962483978444123No Hit
CCAGGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGC256590.13913681168313247No Hit
GTGATTTCCAAAAATCAAGCTGGAGATGTTCTTGAGCCTCCAATTGTAGC244320.13248336190195614No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG242800.13165913666419019No Hit
GTTTGCAGGAGGCCTTTAATTGTGAAGAGCGCAATCAGAAAACAGATATG241160.13076984101291642No Hit
CTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCGGAGACTCGAGTGAA240810.1305800523068519No Hit
TGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGG240370.1303414607906565No Hit
CCGGGAGATGCGCTGGAGCAGGGGAGAGATGCCCCTGCGCCTAGTGGCCT238940.12956603836302144No Hit
GAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTG227990.12362836313043132No Hit
TTAAGGTTTGCAGGAGGCCTTTAATTGTGAAGAGCGCAATCAGAAAACAG225460.12225646191230774No Hit
CTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCGATGTTGT220610.11962653269969935No Hit
CTTTATTCTTTATTCAGGATGTGGGATCGATCAGCACTGCCAGCTGGGCC219010.1187589271862615No Hit
GGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACA218450.11845526525655826No Hit
GGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGGA214050.11606935009460424No Hit
CTTTAATTGTGAAGAGCGCAATCAGAAAACAGATATGCTGGTCAAGGAGG200840.10890618207428318No Hit
GGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGCCCG198380.10757223859737253No Hit
CCATAGTTGAACTTGGTCCCGTAGAGAGAGGCCAGGGCTGTCACAGCAGC189660.10284378854913637No Hit
GTTGGGAAGGATGTCACCAGCGGGAGGGAGTGAGGCGAGCTGGACGGCAT185630.10065850716216485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)