FastQCFastQC Report
Thu 16 Oct 2025
HPAP-138_exocrine_5000068_NSS0004_s_2_CGCAACTA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-138_exocrine_5000068_NSS0004_s_2_CGCAACTA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16611116
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA326060.196290243232303No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG318460.1917149937427443No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT268570.16168088886983872No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG266540.16045881565091713No Hit
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTC261210.1572501209431082No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC201600.12136451277566179No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG192130.11566351110906697No Hit
CGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCA190720.11481468192745148No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG186060.11200933158253785No Hit
GTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTC178790.10763274424186792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)