FastQCFastQC Report
Thu 16 Oct 2025
HPAP-139_alpha_5000069_NSS0004_s_1_CTTACAGC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-139_alpha_5000069_NSS0004_s_1_CTTACAGC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11971963
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC426950.3566248910057607No Hit
CTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTTGCCAG265200.22151755731286507No Hit
GGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGT253110.211418962788308No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT221250.18480678565411537No Hit
CTGCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGG146460.12233582746622254No Hit
CTCAGCAGGGCGGCAATGGTGTAGCGGCGGGGGCCGGAGTCGTTGGCTGT128680.1074844618213404No Hit
GGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTT128530.10735916908530371No Hit
GGCAAGATTATCAAGAATGGTGTTCATCTCATCAGAGAAAGAACCATCAG127570.10655729557466892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)