FastQCFastQC Report
Thu 16 Oct 2025
HPAP-139_alpha_5000069_NSS0004_s_2_CTTACAGC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-139_alpha_5000069_NSS0004_s_2_CTTACAGC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12241984
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC435100.35541624625550894No Hit
CTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTTGCCAG274900.22455510479347135No Hit
GGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGT257170.2100721582384032No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT227180.18557449511451737No Hit
CTGCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGG148210.1210669773788301No Hit
GGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTT136840.11177926715146827No Hit
CTCAGCAGGGCGGCAATGGTGTAGCGGCGGGGGCCGGAGTCGTTGGCTGT134150.10958191090594466No Hit
GGCAAGATTATCAAGAATGGTGTTCATCTCATCAGAGAAAGAACCATCAG127720.10432949430419121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)