FastQCFastQC Report
Thu 16 Oct 2025
HPAP-139_beta_5000070_NSS0004_s_1_TACCTGCA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-139_beta_5000070_NSS0004_s_1_TACCTGCA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15866714
Sequences flagged as poor quality0
Sequence length20-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA460760.2903940916814912No Hit
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGA326050.2054930844534035No Hit
CTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG320630.20207712825730645No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT275120.17339444071406343No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT255930.16129993897917363No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC253530.15978733844953655No Hit
CAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTTTGT252520.15915078572664762No Hit
TTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGAG227920.1436466302978676No Hit
CGATAACGTTGTAGATGTGGTCGTTACCTAGAAGGTTGCCTGGCTGGCCC213140.13433153203618595No Hit
GGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTGC210420.1326172514359306No Hit
GGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTGCC195220.12303744808156244No Hit
GGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGTTAAAAA194950.12286728052197826No Hit
GCCGGATGTCAGAGGGGTGCCTTGGGTAACCTCTGGGACTCAGAAGTGAA183210.11546814293117026No Hit
GCGAAATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTG181560.11442823006704476No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA164170.10346817872938277No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG162970.10271187846456423No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG161780.10196188070195253No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT161380.10170978061367969No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA160790.10133793298347724No Hit
CTGGAGTTTAAGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATA159780.1007013802605883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)