FastQCFastQC Report
Thu 16 Oct 2025
HPAP-139_beta_5000070_NSS0004_s_2_TACCTGCA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-139_beta_5000070_NSS0004_s_2_TACCTGCA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16219369
Sequences flagged as poor quality0
Sequence length20-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA472330.2912135484432224No Hit
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGA336480.20745566612363278No Hit
CTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG324060.19979815490972552No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT284590.17546305284749364No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT266580.16435904504053148No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC260330.1605056275617134No Hit
CAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTTTGT257510.1587669655952707No Hit
TTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGAG233650.14405615902813482No Hit
GGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTGC218660.13481412254693756No Hit
CGATAACGTTGTAGATGTGGTCGTTACCTAGAAGGTTGCCTGGCTGGCCC217500.13409892826286893No Hit
GGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTGCC201780.12440681262014572No Hit
GGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGTTAAAAA201300.12411087015777249No Hit
GCGAAATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTG187750.11575666106369488No Hit
GCCGGATGTCAGAGGGGTGCCTTGGGTAACCTCTGGGACTCAGAAGTGAA186700.11510928692725346No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA166930.10292015675825612No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG166140.10243308478893352No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT165080.10177954518452598No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA164690.10153909193384775No Hit
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG164350.10132946602300003No Hit
CTGGAGTTTAAGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATA163350.10071291922638916No Hit
GGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTTG163320.10069442282249082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)