FastQCFastQC Report
Thu 16 Oct 2025
HPAP-140_exocrine_5000074_NSS0004_s_1_AGACGCTA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-140_exocrine_5000074_NSS0004_s_1_AGACGCTA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20431829
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG1224740.5994274912931191No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA735580.36001671705455246No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT600150.29373288118259017No Hit
CGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCA448680.21959854891111316No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG412100.20169511011471367No Hit
CAGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCT374390.18323861265675237No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT362480.17740947225037954No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT345770.16923105611347863No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC343460.168100467168162No Hit
GAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGAT341640.16720970012033676No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC307240.15037322405155212No Hit
TGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGC296430.1450824593334253No Hit
GCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCAT293970.14387845552152967No Hit
GCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAG292860.14333518550884503No Hit
CGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGG285690.1398259548863687No Hit
GTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTC281680.13786333078649005No Hit
CCATAGTTGAACTTGGTCCCGTAGAGAGAGGCCAGGGCTGTCACAGCAGC271690.13297390067232845No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG270640.13245999660627544No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT269120.1317160592916082No Hit
GAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTG246970.1208751306601088No Hit
CGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCATT238280.11662196272296524No Hit
CGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATTGTCCAGAGTCCGG235150.11509003917368338No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG231200.1131567810204363No Hit
CTGGCTGTAGGTCCAGTCAATAGTGCTTCCACTGGCTTGATAAATTGCCT230760.11294143074513788No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG225640.1104355366325746No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC218390.10688715141458946No Hit
GGCAGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACT216780.10609916517997484No Hit
GGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCAGTGAG211740.10363242566292034No Hit
GTGAAAGCTGCATCCTGCCCGTAGTCTTGAGTGATCTTCTTTGCAAACCA211110.10332408322328852No Hit
CTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCAC204590.1001329836893212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)