FastQCFastQC Report
Thu 16 Oct 2025
HPAP-140_exocrine_5000074_NSS0004_s_2_AGACGCTA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-140_exocrine_5000074_NSS0004_s_2_AGACGCTA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20936337
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG1264200.6038305554596298No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA759790.36290493413437125No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT613570.2930646368560078No Hit
CGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCA465620.22239802502223766No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG422490.20179747775362997No Hit
CAGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCT378370.18072406839840224No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT368950.17622471399844203No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT360340.17211224676026182No Hit
GAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGAT351880.16807142529278163No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC349020.16670537926476825No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC316730.15128243302541414No Hit
TGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGC301610.1440605393388538No Hit
GCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAG298170.14241746299746705No Hit
GCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCAT296490.141615030365627No Hit
CGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGG291400.1391838505465402No Hit
GTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTC288480.1377891462102468No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG280390.13392505097715995No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT277200.13240138425360654No Hit
CCATAGTTGAACTTGGTCCCGTAGAGAGAGGCCAGGGCTGTCACAGCAGC275250.13146998923450648No Hit
GAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTG257860.12316385621801942No Hit
CGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCATT245620.11731756132889913No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG240520.114881605125099No Hit
CGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATTGTCCAGAGTCCGG239680.11448038880917898No Hit
CTGGCTGTAGGTCCAGTCAATAGTGCTTCCACTGGCTTGATAAATTGCCT235550.11250774192257222No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG229820.10977087348183209No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC226300.10808958606273868No Hit
GGCAGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACT224020.10700057034809861No Hit
GTGAAAGCTGCATCCTGCCCGTAGTCTTGAGTGATCTTCTTTGCAAACCA218080.10416339782837848No Hit
GGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCAGTGAG217200.10374307597360512No Hit
CTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCAC211350.100948890916305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)