FastQCFastQC Report
Thu 16 Oct 2025
HPAP-142_beta_5000076_NSS0004_s_1_CAGTGCTT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-142_beta_5000076_NSS0004_s_1_CAGTGCTT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21572300
Sequences flagged as poor quality0
Sequence length20-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGA355020.16457215966772204No Hit
GCCGGATGTCAGAGGGGTGCCTTGGGCAACCTCTGGGACTCAGAAGTGAA297720.1380103187884463No Hit
CAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTTTGT288020.1335138116936998No Hit
TTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGAG238950.11076704848347187No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC233200.10810159324689532No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA232740.10788835682796921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)