FastQCFastQC Report
Thu 16 Oct 2025
HPAP-142_beta_5000076_NSS0004_s_2_CAGTGCTT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-142_beta_5000076_NSS0004_s_2_CAGTGCTT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22097007
Sequences flagged as poor quality0
Sequence length20-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGA367050.16610846889807293No Hit
GCCGGATGTCAGAGGGGTGCCTTGGGCAACCTCTGGGACTCAGAAGTGAA302190.13675607741808654No Hit
CAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTTTGT292900.13255188813580046No Hit
TTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGAG245510.11110554474639937No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA242400.10969811431928314No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC241330.10921388584435893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)