FastQCFastQC Report
Thu 16 Oct 2025
HPAP-144_beta_5000079_NSS0004_s_2_ATAACGCC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-144_beta_5000079_NSS0004_s_2_ATAACGCC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17677865
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG258150.14603007772714635No Hit
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGA253980.1436711955883813No Hit
CAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTTTGT238970.135180351247167No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC219360.1240873827241016No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA216350.12238468842249899No Hit
CGGTGATGTAGAGGGTGATGGTAGATGTGGCGGGTTTTAGGGGCTCTTTG184930.10461104890211571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)