FastQCFastQC Report
Thu 16 Oct 2025
HPAP-144_exocrine_5000080_NSS0004_s_2_GAGAGTAC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-144_exocrine_5000080_NSS0004_s_2_GAGAGTAC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17023812
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG338680.19894486616746002No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT332920.19556137015610842No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC283610.16659605968392976No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC281270.16522151442931818No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT277880.16323018604763728No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG275570.16187326316808479No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG272400.16001116553683745No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA248600.14603074798993315No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA201380.11829312964687344No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT197090.11577313001341885No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC190270.1117669767499782No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG177680.10437145335016622No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC171410.10068837696280951No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG171100.10050627908719857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)