FastQCFastQC Report
Thu 16 Oct 2025
HPAP-146_beta_5000082_NSS0004_s_2_TCTACGCA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-146_beta_5000082_NSS0004_s_2_TCTACGCA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14033921
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTG923790.6582550949232221No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT676410.4819821915771081No Hit
AAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGA525820.37467789650518907No Hit
AGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGT504000.359129854015852No Hit
GGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGG486540.3466885697874457No Hit
CTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT485490.34594038259157933No Hit
AGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAG373790.266347516136082No Hit
AGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAG372170.2651931701767453No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTC363620.25910078872469067No Hit
GTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGT317620.22632306395340263No Hit
TGGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTT287610.20493916133630793No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA267860.1908661164616788No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC262020.18670477053419354No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT258810.18441745539254498No Hit
AGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGC253870.1808974127758023No Hit
CGGGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAG253100.1803487421655003No Hit
AGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGAC244780.1744202493373021No Hit
GGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGG223210.1590503466565046No Hit
GAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGC210750.15017185859889048No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC193860.1381367331339545No Hit
CTGCAGGCTGCCTGCACCAGGGCCCCCGCCCAGCTCCACCTGCCCCACCT189770.1352223658662465No Hit
GAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCA187090.13331270711870188No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG180550.1286525697273057No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT179510.12791150812378094No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA177830.12671440861039476No Hit
TTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTC175640.1251539038875878No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT167380.11926816461343911No Hit
GGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAG160730.11452964570628552No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG160610.11444413859818649No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG159950.11397384950364194No Hit
GCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTG156590.11157965047686959No Hit
CTGCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAG149200.10631383773643872No Hit
CTGGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCT143890.10253014820305742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)