FastQCFastQC Report
Thu 16 Oct 2025
HPAP-146_exocrine_5000083_NSS0004_s_2_GCCTTAAC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-146_exocrine_5000083_NSS0004_s_2_GCCTTAAC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15941988
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG611090.38332107639273094No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT536740.3366832292183384No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG345560.21676092090898574No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA303300.19025230730320458No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA270750.1698345275382217No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC263790.165468698132253No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT250090.15687503967510202No Hit
GGCAGTTGAGGGGTCCTCCAGAGTCACCGTTGCAGCCGGAGCGGATGTAC221970.13923608523604458No Hit
CGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCA191110.11987839910555698No Hit
GCAGGGTGTCTTGTTGGGAAGGATGTCACCAGCGGGAGGGAGTGAGGCGA188970.11853603201808958No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC167190.10487399689423928No Hit
CCAGGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGC162450.10190071652293302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)