FastQCFastQC Report
Thu 16 Oct 2025
HPAP-149_exocrine_5000088_NSS0004_s_1_GAATCACC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-149_exocrine_5000088_NSS0004_s_1_GAATCACC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26071700
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTC689600.264501355876295No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG668050.25623568850516076No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC636890.24428403211144653No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT631040.24204021985524535No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA472040.1810545534046495No Hit
GTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTC464860.17830060947310686No Hit
CTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCAC427410.16393637545691306No Hit
GTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCAC390020.14959515489975722No Hit
GGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCAGTGAG386680.14831407234664407No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT382810.1468297042386956No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG362850.13917389353206733No Hit
GCGGTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAG312230.11975820525704116No Hit
GGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTCA303940.11657851233329625No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA303620.1164557738850938No Hit
CGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCA302150.11589194413866376No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG287530.110284331286414No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC271030.1039556300509748No Hit
GTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAA270350.1036948108485446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)