FastQCFastQC Report
Thu 16 Oct 2025
HPAP-149_exocrine_5000088_NSS0004_s_2_GAATCACC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-149_exocrine_5000088_NSS0004_s_2_GAATCACC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26685853
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTC713550.2673888670525165No Hit
AAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTCCAG685730.2569638677092315No Hit
GTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGC658080.24660257253159568No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT645000.24170109908047532No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA485140.1817966995471346No Hit
GTAAAACTATTGAAGATTAACAAGGCAAACTCAGCAGAGAAGAGAGTGTC471120.17654297953301326No Hit
CTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCAC439790.16480267653426706No Hit
GGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAATCAGTGAG404640.15163090346034658No Hit
GTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCAC402230.1507278032296738No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT394310.14775993857119726No Hit
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG374830.14046019064858073No Hit
GCGGTTCTTTTTGGGGTCATGGAGGCCAATCCAGACATTGAAGTCATCAG319900.11987625053619234No Hit
GGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTCA316400.11856469418459285No Hit
CGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCA313430.11745174493766417No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA310330.1162900807405332No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG297520.1114897844936791No Hit
GTCATGGAGGCCAATCCAGACATTGAAGTCATCAGTGCCACTCTCCTTAA278810.10447857896841446No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC271280.10165685916054472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)