FastQCFastQC Report
Thu 16 Oct 2025
HPAP-152_alpha_5000092_NSS0004_s_1_CAAGGTAC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-152_alpha_5000092_NSS0004_s_1_CAAGGTAC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18681570
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT306250.1639316181669956No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG304850.16318221648394649No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG270610.14485399246423078No Hit
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC237550.12715740700594222No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC231700.12402597854462981No Hit
GTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACC219710.11760788841623053No Hit
TGGCATTATAAGTCCAGCGGGCTGAGCTCCCTGGGGACAGCAGCATGCGG189390.1013779891090524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)