FastQCFastQC Report
Thu 16 Oct 2025
HPAP-152_alpha_5000092_NSS0004_s_2_CAAGGTAC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-152_alpha_5000092_NSS0004_s_2_CAAGGTAC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19127443
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT318250.16638397510843453No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG314160.164245686158887No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG277630.14514747214251272No Hit
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC242630.1268491559483408No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC241420.12621655701705659No Hit
GTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACC228270.11934161821838915No Hit
TGGCATTATAAGTCCAGCGGGCTGAGCTCCCTGGGGACAGCAGCATGCGG195950.10244443023565669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)