FastQCFastQC Report
Thu 16 Oct 2025
HPAP-153_alpha_5000095_NSS0004_s_1_GTCGTTAC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-153_alpha_5000095_NSS0004_s_1_GTCGTTAC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18179570
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG733840.40366191279551716No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG520060.28606837235424165No Hit
GGGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAG493740.27159058217548604No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG474320.26090826130651057No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC452730.24903229284301004No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC379030.2084922800704307No Hit
GGAGGCTTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCA376080.2068695794234957No Hit
TGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGG336150.18490536354820272No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT332900.18311764249649468No Hit
CTTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCAGCTCG322020.17713290248339209No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG316050.17384899642840837No Hit
GGGGAATCCAGCAAAGCCATCAGGGTTGACGATGCTATCAGTGTAATATT300570.16533394354211897No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT297690.16374974765629768No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC296090.16286963883084143No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG288730.1588211382337426No Hit
TTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCAGCTCGT288130.1584910974241965No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC281690.15494865940173502No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG277760.15278689209920807No Hit
ATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAA272800.15005855474029364No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG270240.1486503806195636No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG267560.14717619833692436No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT267510.14714869493612887No Hit
GGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGGA265150.14585053441858087No Hit
TTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAGTTGTAG264010.14522345688044327No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA245380.13497568974403684No Hit
GGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAGT243400.13388655507253472No Hit
AGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTT241990.13311095917010138No Hit
GGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGG241340.13275341495975979No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG240040.13203832653907657No Hit
CGGGACTTGTAGCAGTGGCCTGCTGATACCACCCACTGTTCGTTGATGAG234020.12872691708329737No Hit
GGCGGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACT233480.12842988035470587No Hit
CGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCATT227540.12516247634019947No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC224250.12335275256785501No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT211870.11654291053088714No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA210850.11598184115465877No Hit
CTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCGATGTTGT206790.11374856501006349No Hit
CCAGGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGC201760.11098172289003534No Hit
GGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGCCCG200370.11021712834792022No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC196680.1081873773692117No Hit
GGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACA194810.1071587501794597No Hit
GGATGATCTGGGAGGCTGGCAGCAGGAAGCCATAGCGCCCAGTGTCCCGG193020.10617412843098048No Hit
GGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGA191930.1055745542936384No Hit
CTCGGTGCCAGCAGCTGGAGGGGCAGTGGGCAGAGAGATGGCGGACACGC190540.10480995975152328No Hit
CTCGGAGTCACCCGTGGGGGGCACGGGAGTGGCCTCGGAGTCCCCTGTGG187280.10301673801965613No Hit
GAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGAT187070.102901223736315No Hit
GCAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGC186320.10248867272438238No Hit
GGGAAAACAGGTAGGCGTAGGTCTTGGCACTCTTGGCATTGGCTCTGTGC185480.10202661559101783No Hit
CCATAGTTGAACTTGGTCCCGTAGAGAGAGGCCAGGGCTGTCACAGCAGC184950.10173507954258545No Hit
GGCAAGTTCTTTCACAGTAGCTTTAGCCAGGGCATTCAACTCAGCATTGT182820.10056343466869679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)