FastQCFastQC Report
Thu 16 Oct 2025
HPAP-153_alpha_5000095_NSS0004_s_2_GTCGTTAC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-153_alpha_5000095_NSS0004_s_2_GTCGTTAC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18614211
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG749940.4028857306925338No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG538530.2893112149636641No Hit
GGGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAG509530.27373172035065035No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG481440.258641099534114No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC465560.2501099831736086No Hit
GGAGGCTTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCA391160.21014052113194592No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC390560.20981818676064218No Hit
TGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGG343920.1847620616312988No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT341560.1834942131041708No Hit
CTTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCAGCTCG333310.17906211549874448No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG323110.17358243118658104No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT306420.16461616342481558No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC305890.16433143473016396No Hit
GGGGAATCCAGCAAAGCCATCAGGGTTGACGATGCTATCAGTGTAATATT305170.16394463348459948No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG297280.15970593650195541No Hit
TTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCAGCTCGT290970.15631605336374452No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC289740.15565526790257186No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG289520.15553707863309382No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG280710.1508041356144507No Hit
ATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAA280060.15045494004553833No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT278750.14975117666819185No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG276140.14834902215302062No Hit
TTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAGTTGTAG273320.14683405060789306No Hit
GGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGGA270590.1453674292184611No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA254220.13657307312139097No Hit
AGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTT249330.13394604799526555No Hit
GGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGG248600.13355387451017936No Hit
GGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAGT247380.13289846128852842No Hit
GGCGGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACT242660.1303627642342724No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG241790.129895379395882No Hit
CGGGACTTGTAGCAGTGGCCTGCTGATACCACCCACTGTTCGTTGATGAG238990.12839115232979792No Hit
CGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCATT238280.12800972332375515No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC230610.12388921561058913No Hit
CGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCAGGATGTCATT219660.11800661333429605No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA215240.11563208346569188No Hit
CTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCGATGTTGT213170.11452002988469401No Hit
GGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGCCCG208170.11183391012382958No Hit
CCAGGAAGATGTCCAAGGTGTCGAGAATGGCGGTGAAAGCTGCATCCTGC206370.11086690700991839No Hit
GGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACA201280.1081324370933584No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC201280.1081324370933584No Hit
GGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGA197970.10635422581166615No Hit
CTCGGTGCCAGCAGCTGGAGGGGCAGTGGGCAGAGAGATGGCGGACACGC197560.10613396399127527No Hit
GGATGATCTGGGAGGCTGGCAGCAGGAAGCCATAGCGCCCAGTGTCCCGG197020.10584386305710192No Hit
GAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGAT194580.10453303661380006No Hit
CTCGGAGTCACCCGTGGGGGGCACGGGAGTGGCCTCGGAGTCCCCTGTGG193920.10417846880536596No Hit
CCATAGTTGAACTTGGTCCCGTAGAGAGAGGCCAGGGCTGTCACAGCAGC193400.10389911235023606No Hit
GCAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGC189150.1016159105535013No Hit
GGGAAAACAGGTAGGCGTAGGTCTTGGCACTCTTGGCATTGGCTCTGTGC188420.10122373706841509No Hit
GTTAAAAGTGTCGGTGGAGCGCGCCCGGGACCGGAAGGCGAACATCTGCT187150.10054146264915553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)