FastQCFastQC Report
Thu 16 Oct 2025
HPAP-153_beta_5000096_NSS0004_s_1_GTAAGGTG_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-153_beta_5000096_NSS0004_s_1_GTAAGGTG_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13671496
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG512230.3746700434246552No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG312550.22861433745070764No Hit
GGGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAG307750.22510338298018007No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC279380.20435217916166598No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG266900.19522369753829427No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC250750.183410798642665No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT222770.16294485987488128No Hit
TGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGG221660.16213295165357178No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT209450.15320196121916724No Hit
GGAGGCTTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCA206740.15121973484101522No Hit
CTTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCAGCTCG206510.15105150160596909No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG198080.14488538781710503No Hit
GGGGAATCCAGCAAAGCCATCAGGGTTGACGATGCTATCAGTGTAATATT195940.14332008728232815No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG183720.13438178235944334No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG177930.13014669352936942No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG173280.1267454563860458No Hit
ATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAA172180.12594086265321658No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG169650.12409029706770935No Hit
TTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAGTTGTAG167510.12252499653293246No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC167260.12234213432092582No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT159800.11688552591464753No Hit
AGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTT156000.11410602029214652No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC155800.1139597305225412No Hit
TTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCAGCTCGT155240.11355011916764632No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA153730.11244563140712618No Hit
GGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAGT152320.11141428853140871No Hit
GGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGGA150620.11017082548976352No Hit
GGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGG148920.10892736244811833No Hit
CGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCATT141500.1035000119957611No Hit
CGGGACTTGTAGCAGTGGCCTGCTGATACCACCCACTGTTCGTTGATGAG139450.10200054185730663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)