FastQCFastQC Report
Thu 16 Oct 2025
HPAP-153_beta_5000096_NSS0004_s_2_GTAAGGTG_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-153_beta_5000096_NSS0004_s_2_GTAAGGTG_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14032579
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGGAATTGGCAAACTTGCCGTGGTAAGTCTCCGAGCAGGGGTTACTG521000.37127886470477023No Hit
GGATGATCTTGGCTGCATTGATGAACTGCTCATTCCCCTCCAGGACTTCG325700.23210273749394178No Hit
GGGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAG317760.22644447610093626No Hit
CGTTGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACC287650.20498726570504253No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG272880.19446175931024512No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCCGCCGCC259810.18514771946054961No Hit
CAGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCT230580.16431762115859103No Hit
TGATGAGGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGG226640.1615098692834724No Hit
AGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTT217050.15467577271433855No Hit
CTTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCAGCTCG213880.15241674392141316No Hit
GGAGGCTTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCA212660.15154733851845764No Hit
GGGGAATCCAGCAAAGCCATCAGGGTTGACGATGCTATCAGTGTAATATT201080.14329511346417503No Hit
GGATGATCTTGGCTGCATTGATGAACTGTTCATTCCCCTCCAGGACTTCG200050.14256110726331916No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG191150.13621872358602077No Hit
GCCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGG181790.1295485313141654No Hit
ATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAA178960.12753179583026042No Hit
CTTGGTTCTAGTGGCTTGCTATGGTCTCCTCAATCCAGTCGATGAAGGCG175670.12518725175179846No Hit
ATGGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAG175340.12495208471657276No Hit
TTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAGTTGTAG172890.12320614763686705No Hit
CGCTGATGAGGGAGCCACCGCAGAAGTGGTAGCCAGAATTCAAGGACACC171110.12193767090140736No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT162400.11573068642620861No Hit
TTCACACTTAGCCTGGCTCAGCACAGGAGCGTCCAGGCACTGCAGCTCGT161540.11511782687986293No Hit
AGAGAGATGGTGGACACGCGGGCGTTGATTACTGCACGTGAGGAGAGCTT161300.1149467963087897No Hit
CCGGTTTTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCGGGGACGGC160520.11439094695280176No Hit
GGCGGACACGCGGGAATTGATGACGGCAGGTGAGGAGAGCTTGATCAGCA157250.11206065542192921No Hit
GGGGCTTTAGCTGTTGGCAGCTATGGTGTTCTTAATCCATTTCACATAGT156320.11139791195902052No Hit
GGGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGG153100.10910325179712155No Hit
GGAGCCACCACAGAAGTGGTAGCCAGAATTCAGGGACACCTGGTAGGGGA151600.10803431072791395No Hit
CGGGCGTTGATTACTGCACGTGAGGAGAGCTTGATTAACATGATGTCATT148230.10563275645909423No Hit
CGGGACTTGTAGCAGTGGCCTGCTGATACCACCCACTGTTCGTTGATGAG143360.10216226112106691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)