FastQCFastQC Report
Thu 16 Oct 2025
HPAP-155_alpha_5000147_NSS0022_s_1_CCGCTTAA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-155_alpha_5000147_NSS0022_s_1_CCGCTTAA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72034307
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT5717960.7937828845913656No Hit
CTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAAC2252600.3127121081348086No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT1840810.2555462912970066No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG1780570.2471836093321478No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1671210.2320019542910297No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC1584530.21996879903349387No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA1481080.20560758639629864No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1423900.1976697020212883No Hit
GGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGT1419350.1970380585461869No Hit
CTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTTGCCAG1249110.17340487498547047No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG1245230.17286624274736204No Hit
CTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAATG1174510.16304869844864336No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1172660.1627918763763494No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA1098680.15252176993942623No Hit
CTCAGAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCA1025120.14230996905405086No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT996410.13832436813753202No Hit
GTGGAATAGGAGTAGGGGCTCAGCAGGGCGGCAATGGTGTAGCGGCGGGG959250.13316571505296776No Hit
CTGCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGG947740.13156786529507392No Hit
GGGAACGTTGCCAGCTGCCTTGTACCAGCATTACAAATAATCCAGCCACA944920.13117638516325283No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC877840.12186415564461528No Hit
CGACAGCCGTGGTGGAATAGGAGTAGGGGCTCAGCAGGGCGGCAATGGTG870220.1208063263522477No Hit
GGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTA841750.11685404289375617No Hit
GGCAAGATTATCAAGAATGGTGTTCATCTCATCAGAGAAAGAACCATCAG821570.11405259996462519No Hit
GGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTTGAAGGGAACGTT806110.1119064003766983No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT782010.10856077229978765No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG765810.10631184388294317No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT759470.10543170770005465No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC742460.10307033286236793No Hit
GCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCT737790.10242203065825288No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT735380.10208746785056182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)