FastQCFastQC Report
Thu 16 Oct 2025
HPAP-155_exocrine_5000149_NSS0011_s_1_CAATGCGA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPAP-155_exocrine_5000149_NSS0011_s_1_CAATGCGA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22969506
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG866750.3773481240737176No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA438070.19071807639223934No Hit
CTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCGG408690.1779272048776321No Hit
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGA373700.16269396477225065No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA368780.16055199445734705No Hit
GTCGATTTGGCGAGGGCGCTCCCGACGACGCACCGGGAGGAGGCCCTTCC363460.15823588021440252No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA341510.1486797321631558No Hit
CTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG338970.1475739182200958No Hit
GCGAAATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTG302510.13170069917916388No Hit
CGGTGATGTAGAGGGTGATGGTAGATGTGGCGGGTTTTAGGGGCTCTTTG300870.13098670907419604No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT295560.1286749484294525No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC293460.12776069280723756No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC270530.11777789213229053No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC257190.11197019213212508No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT257070.11191794895371282No Hit
GTTTATTATAATAATGTTTGTATATTCGGCTATGAAGAATAGGGCGAAGG244940.10663703433587123No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG236920.10314544857865032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)